Postdoctoral Researchers will be recruited for the following bio-imaging projects focused on molecular, cellular, organelle and organism levels, respectively:
Project 9: Laboratory of Plasma Membrane Receptors (Prof. Andrzej Sobota/Prof. Katarzyna Kwiatkowska)
A new post-doc will work on imaging of raft-based signaling complexes assembled during activation of plasma membrane receptors of immune cells (Szymanska et al., Eur. J. Immunol. 2008; Kulma et al., BBA-Biomembranes, 2010; Jozefowski et al., J. Immunol. 2010). The studies will focus on co-receptors, distinct adaptor proteins and signaling lipids recruited by the receptors in the course of activation. The research will involve cellular and molecular biology combined with confocal, immunoelectron and TIRF microscopy.
Project 12: Laboratory of Animal Models (dr Witold Konopka)
A postdoctoral researcher will participate in starting a new, fully equipped set-up that allows for in vivo introduction of optogenetical tools into the brain and light-stimulated manipulation of those. The Laboratory of Animal Models has extensive expertise in generation of animal models with precise, local viral vectors-mediated genetic manipulation, especially in the brain (Konopka et al.,Neurobiol Dis. 2005 Jun-Jul;19(1-2):283-92.; Konopka et al., Genesis. 2009 Apr;47(4):274-80.). The project performed by successful candidate will be related to variety of brain structures like: amygdala, cortex or hippocampus, in which the activity can be induced or suppressed by optogenetical light-responsive receptors.
Project 1: Laboratory of Cell Signaling and Metabolic Disorders (Prof. Agnieszka Dobrzyn)
A postdoctoral researcher will be involved in a project on metabolic regulation of pancreatic beta-cell function (Dobrzyn et al. PNAS 2004; Dobrzyn et al. Am. J. Physiol. 2008, Dobrzyn et al. J. Lipid. Res. 2010). Using fluorescent and confocal microscopy and flow cytometry techniques the postdoc will investigate signaling pathways involved in beta-cell development and insulin secretion in different animal models of diabetes (Dobrzyn et al. Biochim. Biophys. Acta 2010).
Project 2: Laboratory of Bioenergetics and Cellular Membranes (Prof. Jerzy Duszynski)
A postdoctoral researcher will participate in studies concerning effects of mitochondrial stress on the cell. In this field the laboratory have a considerable experience (Giorgi et al. Science, 2010; Wojewoda et al. Bioch. Biophys. Acta - Bioenergetics 2010; Lebiedzinska et al. Bioch. Biophys. Acta - Bioenergetics 2010; Wieckowski et al. Nat Protoc. 2009). The studies on physiology and visualization of mitochondria in situ will be performed on animals and cells lines relevant to neurodegenerative and metabolic diseases.
Project 3: Laboratory of Physiology of Cell Movements (Prof. Stanislaw Fabczak)
The postdoctoral researcher will use advanced methods of bioimaging and molecular biology to investigate the localization and function of phosducin-like proteins (PhLPs), PhLPs interacting proteins and ciliary proteins newly identified in a ciliate Tetrahymena or cultured epithelial cells exploring their role in cilia assembly, maintenance and function. A strong background in molecular biology and experience with ciliate and / or mammalian cells culture will be an advantage although is not required (see: Sobierajska et al. Photochem. Photobiol. Sci. 2010, Wloga et al., J. Cell, Sci. 2010, Wloga et al. Dev. Cell 2009).
Project 4: Laboratory of Molecular Neurobiology (Prof. Leszek Kaczmarek)
A newly hired post-doc will study synaptic plasticity, focused on extracellular matrix metalloproteinase 9 (MMP-9), and proteins regulating its functions. He/she will perform quantitative spatial and temporal analyses of MMP-9 interactome that will be complemented by high-resolution confocal microscopy or electrophysiology-based methods, and will establish additional strategies allowing investigations of the effects of MMP-9 and its protein partners on synaptic plasticity in vitro and in vivo (see: Konopka et al. J. Neurosci. 2010; Michaluk et al. J. Neurosci. 2009; Wilczynski et al., J. Cell Biol. 2008; Okulski et al. Biol. Psych. 2007).
Project 5: Laboratory of Transcription Regulation (Prof. Bozena Kaminska)
A postdoctoral researcher will perform multimodal (optical, magnetic and radioactive reporter) imaging of microglia-glioma interactions in an intracranial glioma model (see: Sliwa et al. Brain 2007; Wesolowska et al. Oncogene 2008; Walzlein et al. PNAS 2009) using various knockout and GFP transgenic mice. A postdoc will further characterize molecular mechanisms activated in tumor-infiltrating microglial cells using high-throughput transcriptomics and chIP-chip/chIP-sequencing technologies.
Project 6: Laboratory of Neuroplasticity (Prof. Malgorzata Kossut)
A newly employed post-doc will be involved in studies aimed to investigate instability of cortical network of inhibitory neurons in the somatosensory cortex of mice following sensory training. Experiments will involve characterization of subpopulations of GAD-immunoreactive neurons after training, followed by investigating effects of siRNA inhibition of GAD67 expression upon cortical plasticity. Additionally, imaging of axonal endings of cortical interneurons in multiphoton microscopes will be attempted in genetically modified mice expressing GFP under GAD promoter (Siucinska & Kossut, Cerebral Cortex 1996; Jasinska et al. J.Neurosci. 2010; Urban-Ciecko et al. J.Neurophysiol. 2010).
Project 7: Epileptogenesis Laboratory (Prof. Katarzyna Lukasiuk)
A postdoctoral researcher will use fluorescence and confocal microscopy to investigate the role of the new epileptogenesis-related gene Ttyh1 in structural plasticity (neuronal branching and synaptogenesis) in in vitro model (Stefaniuk et al. J. Neurochem. 2010). Previous experience in neuroscience is required.
Project 8: Laboratory of Molecular Bases of Ageing (Prof. Ewa Sikora)
The hired post-doc will use flow and scanning cytometry, confocal and electron microscopes as well as video time laps technique to investigate the propensity of curcumin to induce senescence of cancer and normal cells (Sliwinska et al, Mech. Ageing Dev., 2009, Belak-?mijewska et al, Cell Prolif., 2010) . Curcumin is an anticancer agent, however the molecular pathways leading to senescence induced by curcumin are poorly recognized (Sikora et al. Curr. Pharm. Des., 2010).
Project 10: Laboratory of Molecular and Systemic Neuromorphology (Prof. Grzegorz Wilczynski)
A newly hired post-doc will study the role of CD44 adhesion and signaling molecule in synaptic plasticity (Gorlewicz et al., Neurobiol. Dis. 2009). He/she will perform studies in neuronal cultures, by means of siRNA techniques, expression constructs, and functional approaches, with the read-out by high-resolution confocal microscopy and quantitative image analysis. In addition the post-doc will do RNA/protein expression analyses using high-throughput techniques.
Project 11: Laboratory of Functional and Structural Tissue Imaging (Dr Tytus Bernas)
A post-doctoral researcher will develop novel superresolution optical imaging technology for structural and functional study of brain tissue /in vitro/ and /in vivo/. The project will involve construction of microscopy equipment and development of novel imaging methods, based on adaptive optics, to elucidate physiology and architecture of rodent anterior brain region. The researcher will apply his or her practical experience in one (or more) of the following fields: biomedical optical imaging, interferometry, superresolution optical microscopy, adaptive optics, Raman spectroscopy, optical spectroscopy, pump probe spectroscopy or optical coherence tomography. The position will suit a person with PhD in: physics, optical engineering, electronic engineering or related discipline.